PHYTOCHEMICALS SCREENING AND EXTRACTION FROM Artemisia vulgaris L.

Abstract

Mugwort (Artemisia vulgaris) is a very common medicinal herb that contains many bioactive compounds, making it one of the plants with great potential in the field of medicine and herbal medicine. The current study aimed to identify and quantify bioactive compounds of mugwort extract using UV-Vis spectrophotometry. Additionally, the conditions affecting the extraction of bioactive compound-rich extracts were also identified through microwave - assisted extraction methods. The results showed that mugwort extract contained phenolics, flavonoids, saponins, carotenoids, steroids and tannins. The total phenolics, flavonoids, saponins and tannins content was found to be a maximum in stems + leaves extract of 14.977 mgGAE/g dry mass, 5.271 mgQE/g dry mass, 3.091 mg/g dry mass and 2.797 mgGAE/g dry mass, respectively. The total steroid content was found to be a maximum in stem extract of 1.439 mg/g dry mass. The carotenoids content was found to be a maximum in the leaves extract of 2.497 mg/g dry mass. The suitable microwave conditions to obtain the highest TPC, TSC, and TFC from leaves, stems, and stems+leaves were 360 W for 2.5 minutes, 540 W for 3 minutes and 540 W for 2.5 minutes, respectively.