OPTIMIZATION OF LECTIN EXTRACTION BY ENZYME-ASSISTED METHOD FROM ALGAE Chaetomorpha linum

Abstract

Chaetomorpha linum is a brackish alga widely distributed in the Mekong Delta. This study used the cellulase enzyme to enhance lectin extraction from C. linum. Response surface methodology (RSM) was employed to investigate the impact of enzyme concentration (0, 0.5, 1, 1.5, 2, 2.5%, w/w), temperature (30, 40, 50, 60 °C), and extraction time (2, 4, 6, 8 hours) on lectin extraction from C. linum algae. The RSM method was employed to optimize the conditions for enzyme-assisted lectin extraction. The obtained optimal extraction conditions were enzyme concentration of 2.55% w/w, temperature 49.98 °C, and extraction time of 6.9 hours. Under the optimum conditions, the obtained protein content of 0.134 g/g dry mass had the highest lectin activity of 61946.79 HU/g. The enzyme-assisted extraction technique has proven to be an efficient approach for enhancing the extraction yield of lectins from C. linum.