OPTIMIZATION OF TRITERPENES EXTRACTION FROM Ganoderma lucidum BY SUPERCRITICAL CARBON DIOXIDE

Abstract

Ganoderma lucidum is one of the popular mushrooms in Asia. Nowadays, Ganoderma lucidum is used all over the world as a tonic or functional food. The bioactive properties of Ganoderma lucidum are mainly from polysaccharides, peptidoglycan, and triterpenes. Water is normally used to obtain Ganoderma lucidum liquid. However, this traditional method only collects the polarized active substance as a polysaccharide. In recent years, the extraction by the supercritical solvent to improve the resulted extraction and assure the biological actives quality has been mentioned in some research. In this study, we used supercritical CO2 for Ganoderma lucidum extraction. The results showed that the best condition to extract with supercritical CO2 was by ethanol 14% (w/w) with a flow rate of 14 g/min for 120 mins, at 59 oC temperature and 153 bar pressure. At this condition, the triterpene yield extraction and the total soluble dry matter were 88.9% and 39.2%, respectively. As compared to Ganoderma lucidum prepared by enzyme assistance, the supercritical CO2 method gave a higher extraction yield. However, the extraction of the total soluble dry matter was not higher than by applying the enzyme method.