OPTIMIZING ALLOPHYCOCYANIN EXTRACTION FROM Chaetomorpha sp. ALGAE WITH ENZYME CELLULASE

Abstract

Allophycocyanin (APC) is a pigment-carrying protein belonging to the phycobiliprotein group, with many specific biological activities. This study was carried out to obtain APC from Chaetomorpha sp. with the support of cellulase enzyme by experimental method. The surveyed influencing factors include enzyme treatment time (30; 60; 90; 120; 180 min), material/solvent ratio surveyed (1:10; 1:15; 1:20); 1:25; 1:30, w/v), and enzyme/substrate ratio (1%; 2%; 3%; 4%; 5%). Response surface methodology (RMS) was performed to optimize the factors affecting the extraction process. The analysis results of JMP 10 software showed that the optimal APC content was 0.27 mg/mL with an enzyme/substrate ratio of 3.21%; the material/solvent ratio is 1:20.89 w/v; extraction time 102.68 min.