MULTIRESIDUE ANALYSIS FOR TWENTY-FOUR POLYCYCLIC AROMATIC HYDROCARBONS IN EDIBLE OILS BY ULTRASOUND-ASSISTED EMULSIFICATION MICROEXTRACTION AND SOLID-PHASE EXTRACTION CLEAN-UP FOLLOWED BY GC-MS/MS

Tóm tắt

In this study, an analytical method for simultaneous determination of 24 polycyclic aromatic hydrocarbons (PAHs) comprising of 16 Environmental Protection Agency (EPA) priority PAHs and 15 1 European Union (EU) priority PAHs in edible oil was proposed by gas chromatography-tandem mass spectrometry (GC-MS/MS). An efficient ultrasound-assisted emulsification microextraction (USAEME) method was adopted by using dimethyl sulfoxide (DMSO) as extracting solvent followed by solid-phase extraction (SPE) clean-up. Under optimum conditions, the calibration curves showed a good linearity in the range of 1 to 50 μg/L with correlation coefficients greater than 0.995. The limit of detections (LODs) for 24 PAHs were 0.12 – 0.32 μg/kg. Using the spiked oil sample at three levels, the mean recoveries were 81 – 115% and the relative standard deviations (RSDs) in term of reproducibility were below 17.7%. Trueness assessment was further participated in international olive oil proficiency test (FCCE1-OIL22-06145) organized by the Food Analysis Performance Assessment Scheme (FAPAS) with satisfaction result (z-score < 2). The method was successfully applied to real oil samples and the results suggested that an extension to large-scale study on the PAHs contamination in edible oil in Vietnamese market is encouraged.